human osteoblast cell line  (PromoCell)

Journal: Cell Death Discovery

Article Title: Unveiling the toxic effects of perfluorooctanoic acid on osteoblast function and extracellular matrix deposition using 2D and 3D models

doi: 10.1038/s41420-025-02863-5

Figure Lengend Snippet: a , c Representative brightfield images of hFOB1.19 spheroids after 2 and 5 days of exposure to PFOA, respectively. Spheroids exposed to 0.02% of vehicle (DMSO) were used as the control. Images were obtained using the Light Lionheart FX Microscope. Scale bar = 200 μm (magnification 10×). b , d Morphological parameters of hFOB1.19 spheroids after 2 and 5 days of exposure to PFOA, respectively. ImageJ software determined area, solidity, circularity, and roundness after 2 and 5 days of exposure to different PFOA concentrations ( n = 31–40), individual biological replicates. P values indicate the results of comparisons between each PFOA-treated group and the control group. Values of p < 0.05 are considered statistically significant.

Article Snippet: The human osteoblast cell line hFOB1.19 (ATCC CRL-11372) was obtained from the American Type Culture Collection (ATCC, USA) in March 2021.

Techniques: Control, Microscopy, Software

Journal: Cell Death Discovery

Article Title: Unveiling the toxic effects of perfluorooctanoic acid on osteoblast function and extracellular matrix deposition using 2D and 3D models

doi: 10.1038/s41420-025-02863-5

Figure Lengend Snippet: Western blot analysis of oxidative stress marker expression, a (60 kDa) CAT and b (96 kDa) NRF2 for 2D ( n = 5 biological replicates). c – e CAT levels in hFOB1.19 spheroids exposed to different PFOA concentrations for 2 days ( c ) and 5 days ( e ). NRF2 levels in hFOB1.19 spheroids exposed to different PFOA concentrations for 2 days ( d ) and 5 days ( f ) ( n = 3–4 individual biological replicates). P values indicate the results of comparisons between each PFOA-treated group and the control group. Values of p < 0.05 are considered statistically significant.

Article Snippet: The human osteoblast cell line hFOB1.19 (ATCC CRL-11372) was obtained from the American Type Culture Collection (ATCC, USA) in March 2021.

Techniques: Western Blot, Marker, Expressing, Control

Journal: Cell Death Discovery

Article Title: Unveiling the toxic effects of perfluorooctanoic acid on osteoblast function and extracellular matrix deposition using 2D and 3D models

doi: 10.1038/s41420-025-02863-5

Figure Lengend Snippet: Western blot analysis of COL1A2 and CB1 levels. a , b (120 kDa) COL1A2 and (53 kDa) CB1 respectively for 2D ( n = 5 individual biological replicates). c – e Degraded COL1A2 (60 kDa) levels in hFOB1.19 spheroids exposed to different PFOA concentrations for 2 and 5 days, respectively. d , f CB1 levels in hFOB1.19 spheroids exposed to different PFOA concentrations for 2 and 5 days, respectively ( n = 3–4 individual biological replicates). P values indicate the results of comparisons between each PFOA-treated group and the control group. Values of p < 0.05 are considered statistically significant. g Localization of CB1 on the osteoblast membrane on the spheroid surface. Scale bar: 20 μm. Image captured using confocal microscope LSM 800 (Zeiss) (40×).

Article Snippet: The human osteoblast cell line hFOB1.19 (ATCC CRL-11372) was obtained from the American Type Culture Collection (ATCC, USA) in March 2021.

Techniques: Western Blot, Control, Membrane, Microscopy

Journal: Biomedicines

Article Title: CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development

doi: 10.3390/biomedicines13123039

Figure Lengend Snippet: Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

Article Snippet: Normal human fetal osteoblast cell line (hFOB 1.19, ATCC CRL-11372) was cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F12 Medium, supplemented with 2.5 mM L-glutamine, 0.3 mg/mL G418 (Sigma-Aldrich Chemicals, Warsaw, Poland), and 10% FBS (Pan-Biotech GmbH, Aidenbach, Bavaria, Germany) and maintained at 34 °C (5% CO 2 in air atmosphere).

Techniques: MTT Assay, Cell Culture, Negative Control, Control, Laser-Scanning Microscopy, Staining, Fluorescence